EKSPLORASI FAGE LITIK SPESIFIK Escherichia coli PADA PERAIRAN SUNGAI BUAH KECAMATAN ILIR TIMUR II KOTA PALEMBANG

Warsa, Kasih (2021) EKSPLORASI FAGE LITIK SPESIFIK Escherichia coli PADA PERAIRAN SUNGAI BUAH KECAMATAN ILIR TIMUR II KOTA PALEMBANG. Undergraduate Thesis thesis, UIN RADEN FATAH PALEMBANG.

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Abstract

Bacteriophage is a virus that attacks its host bacteria as a target by destroying bacterial cells through the lysis process so that the host bacteria dies. The use of this bacteriophage is often used as a natural alternative method that is environmentally friendly. The purpose of this study was to determine the number of specific lytic phage Escherichia coli isolated from the waters of Buah river, Ilir Timur II District, Palembang City. This study used a quantitative descriptive experimental method with parameters, namely isolating Escherichia coli bacteria, gram staining, pathogenic testing and isolating specific Escherichia coli lytic phages. The samples tested were 9 samples taken from the waters of Sungai Buah, Ilir Timur II District, Palembang City, it was found that six samples that were positive for Escherichia coli were St 1-2, St 2-1, St 2-3, St 3-2 and St 3-3 some are pathogenic, including St 1-2, St 2-3 beta-hemolytic; St 1-3, St 2-1 and St 3-3 alpha - hemolytic; and St 3-2 gamma-hemolytic which means they are non-pathogenic. The results of bacteriophage isolation showed that nine phage isolates were obtained with codes including FK 1a, FK 2b, FK 3c, FK 4a, FK 5b, FK 6c, FK 7a, FK 8b, and FK 9c that could lysis Escherichia coli cells. This research conclusion that the specific lytic phage of Escherichia coli can be used as a natural biocontrol of water pollution. Keywords: Lytic phage, Escherichia coli, Buah river.

Item Type: Thesis (Undergraduate Thesis)
Uncontrolled Keywords: Lytic phage, Escherichia coli, Buah river.
Subjects: Sains dan Teknologi > Biologi
Divisions: Fakultas Sains dan Teknologi > 46201 - Biologi
Depositing User: UPT PERPUSTAKAAN #3
Date Deposited: 30 Jun 2022 03:16
Last Modified: 30 Jun 2022 03:16
URI: http://repository.radenfatah.ac.id/id/eprint/20855

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